Defining potency of CAR+ T cells: Fast and furious or slow and steady

Defining potency of CAR+ T cells: Fast and furious or slow and steady

Blog Article

Genetically engineered T cells that express chimeric antigen receptors (CAR+) are heterogeneous and here thus, understanding the immunotherapeutic efficacy remains a challenge in adoptive cell therapy.We developed a high-throughput single-cell methodology, Timelapse Imaging Microscopy In Nanowell Grids (TIMING) to monitor interactions between immune cells and tumor cells in vitro.Using TIMING we demonstrated that CD4+ CAR+ T cells participate in multi-killing and benefit from improved resistance to activation induced cell death in comparison to CD8+ CAR+ T cells.For both subsets of cells, effector cell fate sapatilha infantil prata glitter at the single-cell level was dependent on functional activation through multiple tumor cells.